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. 2023 Jun 20;24(12):10384. doi: 10.3390/ijms241210384

Table 3.

Optical filter setup of the multiphoton microscope, considering different sample types and fluorescence staining.

PEM (Figure 3) PEM (Figure 8) GEC (Figure 8) MC (Figure 8)
Dichroic mirrors 495 nm 560 nm 560 nm 560 nm
560 nm n.a. n.a. n.a.
Fluorophores tdTomato 572/35 nm n.a. n.a. n.a.
CFSE 525/50 nm n.a. n.a. n.a.
E450 450/30 nm n.a. n.a. n.a.
Alexa Fluor 647 n.a. 675/67 nm 675/67 nm 675/67 nm