Table 1.
Linear regression for BCR::ABL1 (left panel) and ABL1 (right panel) quantification using RNA from KCL-22 cell line standard curve, where amplification efficiency was 90% and 87%, respectively.
BCR::ABL1 | ABL1 | |||
---|---|---|---|---|
RNA (ng) | Ct (SD) | rRSD% | Ct (SD) | rRSD% |
200 | 17.84 (0.207) | 1.16 | 20.47 (0.771) | 3.766 |
100 | 18.92 (0.063) | 0.333 | 21.62 (0.378) | 1.748 |
50 | 19.94 (0.116) | 0.582 | 22.61 (0.484) | 2.141 |
25 | 21.05 (0.136) | 0.646 | 23.81 (0.505) | 2.121 |
12.5 | 22.16 (0.307) | 1.385 | 24.87 (0.626) | 2.517 |