Figure 12.

Effect of 3,6′-DMC on the phosphorylation expression of MAPK in LPS-induced RAW 264.7 cells. The cells were treated with 3,6′-DMC (2.5, 5, and 10 μM) in the presence of LPS (1 μg/mL) via stimulation for 20 min. (a) Western blotting results and protein expression of (b) p-ERK/ERK, (c) p-p38/p38, (d) p-JNK/JNK. Equal amounts of protein loading were confirmed using β-actin. Data are expressed as the mean $\pm$ SD from a single triplicate experiment using ImageJ software. # p < 0.001 vs. untreated control group. *** p < 0.001 vs. LPS-alone group.