Figure 13.

Effect of 3,6′-DMC on the protein expression of p-IκB-α and IκB-α in LPS-induced RAW 264.7 cells. The cells were treated with 3,6′-DMC (2.5, 5 and 10 μM) in the presence of LPS (1 μg/mL) via stimulation for 15 min. (a) Western blotting results and protein expression of (b) p-IκB-α/β-actin, (c) IκB-α/β-actin. Equal amounts of protein loading were confirmed using β-actin. Data are expressed as the mean $\pm$ SD from a single triplicate experiment using ImageJ software. # p < 0.001 vs. untreated control group. * p < 0.05, *** p < 0.001 vs. LPS-alone group.