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. 2023 Jun 16;24(12):10250. doi: 10.3390/ijms241210250

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Human MSCs and rat Schwann cells grown on OPF+ scaffold sheets with ridges spaced 0.4 mm apart. Live culture images taken at 10× bright-field. (A) Human MSCs attach, but do not spread (blue arrowheads) when grown on ridged OPF+ sheets, whereas the rat Schwann cells (B) attach and spread along the ridges (raised portions of the polymer are indicated by a white arrow). This is not observed on glass coverslips where hMSCs (C) and Schwann cells (D) spread into their stereotypical morphologies.