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X/A ratio determines sex.
| Karyotype | X/A Ratio | Sexual Fate |
|---|---|---|
| 2A;2X | 1 | female |
| 2A;1X | 0.5 | male |
| 3A;3X | 1 | female |
| 3A;2X | 0.67 * | Male |
| 4A;4X | 1 | female |
| 4A;3X | 0.75 * | female |
| 4A;2X | 0.5 | male |
* X/A limits for female and male development. An X/A ratio ≥ 0.75 yields female fates and an X/A ratio ≤ 0.67 promotes male fates.
