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. 2023 Jun 20;24(12):10400. doi: 10.3390/ijms241210400

Figure 6.

Figure 6

COX5A alleviates DOX-induced oxidative stress and cardiomyocyte apoptosis in vitro. (A) Expression of COX5A in H9c2 cells after transfection of lentivirus-COX5A and lentivirus-NC by Western blot. (B) DCFH-DA staining to detect ROS level. Nuclei were counterstained using Hoechst 33,342. Scale bars = 100 μm. (C) The morphology of mitochondria in H9c2 cells were stained with MitoTracker Green probe. (D) Annexin V-FITC staining followed by flow-cytometric analysis. (E) Quantitative analysis of Annexin V positive ratio. (F–I) Western blot analysis of the protein level of SOD2, p-Akt(T308)/t-Akt, and p-Akt(S473)/t-Akt. Data are expressed as mean ± SEM. * p < 0.05.