Figure 7.

PI3K/Akt signaling is responsible for COX5A-mediated protective role in DOX-induced cardiotoxicity. (A) DCFH-DA staining to detect ROS level. Scale bars = 100 μm. (B) Cell viability detected by CCK-8 assay. n = 8. (C) Annexin V-FITC/PI staining followed by flow-cytometric analysis. (D) Quantitative analysis of of Annexin V positive ratio. n = 4. (E) The morphology of mitochondria in H9c2 cells stained with MitoTracker Green probe. (F) OCR measurements by means of the Seahorse^TM XFe96 extracellular flux analyzer at baseline and after addition of oligomycin, FCCP, and rotenone + antimycin A. (G–J) Western blot analysis of the protein level of p-Akt(S473)/t-Akt, cleaved caspase-3/t-caspase-3, and SOD2. Data are expressed as mean ± SEM. * p < 0.05.