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. 2023 Jun 16;24(12):10209. doi: 10.3390/ijms241210209

Figure 3.

Figure 3

IGF-1 or Insulin do not enhance ECAR or OCR response in MCF-7L TamR cells. Tx indicates 5 nM IGF-1, 10 nM insulin, and Vehicle injection (Seahorse DMEM media supplemented with glucose (10 mM), L-glutamine (2 mM), and sodium pyruvate (1 mM)). (A,B) MCF-7L TamR cells were analyzed using the Seahorse Xfe96 analyzer. No change was observed in the ECAR and OCR readings when these cells were stimulated with 5 nM IGF-1 and 10 nM insulin as compared to vehicle (Seahorse DMEM media supplemented with glucose (10 mM), L-glutamine (2 mM), and sodium pyruvate (1 mM)). A p-value < 0.05 was considered significant.