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. 2023 Jun 18;24(12):10294. doi: 10.3390/ijms241210294

Figure 2.

Figure 2

RECs/BMSCs donate mitochondria to ρ0 cells via tunneling nanotubes (TNTs). (A) Representative F-actin immunofluorescence images of RECs cocultured with ρ0 cells. (B) Representative F-actin immunofluorescence images of BMSCs cocultured with ρ0 cells. Mito-Red labeled RECs or BMSCs, and F-actin (green) and Hoechst33342 (blue) labeled all the cells. White arrows: mitochondria of RECs or BMSCs transferred into microtubules. Scale bars, 100 µm. (C) Representative immunofluorescence images of RECs (I) or BMSCs (II) cocultured with ρ0 cells after the addition of Cyto-D (a potent inhibitor of actin polymerization). Mito-Green labeled RECs or BMSCs, Hoechst33342 (blue) labeled ρ0 cells, and F-actin (red) labeled all the cells. Scale bars, 100 µm. (D) Relative rates of mitochondrial transfer from RECs and BMSCs to ρ0 cells with and without Cyto-D treatment (n = 3). (E) Number of TNTs between REC/BMSCs and ρ0 cells with or without Cyto-D treatment (n = 3). Cyto-D: Cytochalasin D. Data represent the mean ± standard deviation of three independent experiments. Ns, not significant. * p < 0.05, *** p < 0.001.