Table 2.

Comparison of analytical properties of the current NZ-based CHOL bionanosensors with known analogs.

Biosensors	Sensitivity, A·M−1·m−2	LOD µM	Linear Range, mM	KMapp, mM	Potential, V	Redox Agent	Reference
GlOx/nCeAu/GCE	33 ± 2	4	0.05	1.1	−0.25	nCeAu NZs	This work
GlOx/nAuHCF/GCE	63 ± 4	4	0.18	0.2	−0.25	nAuHCF NZs	This work
ChOx/nCuFe/GCE	321 ± 12	2.8	0.003–0.1	0.85	−0.25	nCuFe NZs	This work
GlOx/nPt/GCE	305 ± 14	1.7	0.02–0.4	2.46	−0.25	nPt NZs	This work
ChOx/nCuFe/nPt/GCE	3960 ± 20	0.2	0.002–0.05	0.41	−0.25	nCuFe/nPt NZs	This work
Ti/NPAu/ChOx–HRP–ChE	29.33	0.01	0.97–7.80	0.64	-	HRP	[60]
Au-f-MWCNT-PPy-ChOx/GCE	101.2	-	2–8	1.66	0.6	K4[Fe(CN)6]	[37]
ChOx/Graphene/PVP/PANI	347.7	-	0.05–0.10	-	0.6	PANI	[45]
SPE/PANi/CNC/IL/GLU/ChOx	351.9	-	1–12	-	0.6	PANI	[46]
ChOx/CS/ZnO@ZnS/GCE	526.7	20	0.4–3	-	0.3	K4[Fe(CN)6]	[19]
ChOx/TH/Cu2O/GCE	702	0.0018	0.01–1	0.025	-	Thionine	[26]
ChOx/Fe3O4@PAMAM	739	-	0.1–1.5	-	0.6	K4[Fe(CN)6]	[64]
ChOx/Fe3O4@APTES	1019	-	0.1–1	-	0.6	K4[Fe(CN)6]	[64]
ChOx/AuNP/carbon IDE	1203	24.6	1–10	-	0.6	K4[Fe(CN)6]	[62]
Pt-NC/enzyme/Nafion	1320	2	0.02–0.48	-	0.6	Pt-NC	[63]
ChOx/MoS2-AuNP/GCE	44,600 (4460 μA mM−1 cm−2)	0.026	0.05–0.048	0.325	-	MoS2-AuNPs	[56]
SPE-PEDOT-ChOx-nafion	1.34 μA·mM−1	-	0.05–0.8	0.24	–0.51	PEDOT	[51]
ChOx/KMWNTs/GCE	2 μA·mM−1	-	0.005–0.016	-	0.6	KMWNTs	[65]
ChOx/CeO2-NR	858	680	1–6.5	0.68	1.25	CeO2	[48]
Ag/ChOx/ChE/Au NPs/SPE	336	0.009	0.01–15	-	-	Ag	[66,67]

ChOx—cholesterol oxidase; HRP—horseradish peroxidase; ChE—cholesterol esterase; TH–thionine; APTES—3-aminopropyltriethoxysilane; PAMAM—polyamidoamine dendrimer; PANi—polyaniline; CNC—Crystalline nanocellulose; NPAu—gold nanoparticle; Pt NC—nano-cluster; GLU—glutaraldehyde; NR—nanorods; KMWNTs—potassium-doped multi-walled carbon nanotubes; PVP—polyvinylpyrrolidone; PPy—polypyrrole; PEDOT—poly-3,4-ethylenedioxythiophene; GCE—glassy carbon electrode.