Figure 5.

Effect of fluorinated benzofuran and dihydrobenzofuran on inflammation in the zymosan-treated sterile air pouch model in mice. (A) A timeline for the zymosan-induced inflammation experiments of the air pouch model in C57/BL6 mice. The pouch was formed on day 1 and refilled on day 4 with sterile air. Mice were injected at day 6 with 1% zymosan (w/v in saline), or 0.5 mL of saline for control mice. An amount of 50 nmol of compounds 2, 3, 5, and 6, or vehicle, were co-injected into the air pouch. At 24 h post-treatment, exudates were collected. (B) Recruited cells in the pouch and PGE₂ production. (C) Gene expressions of inflammatory enzymes Ptgs2 and Nos2, proinflammatory cytokines Il1α and Il1β, and chemokines Ccl3 and Ccl4. Data correspond to the mean ± SEM (n = 6–8 mice per group); * p < 0.05 compared to the zymosan-treated cells (one-way ANOVA followed by Dunnett’s test).