Fig. 5.

Transcript levels of flowering time genes in Bowman, BW290, and HIF pairs 10_003 and 10_190. Diurnal gene expression of HvELF3 (A), HvCO1 (B), HvGI (C), PPD-H1 (D), HvFT1 (E), and VRN-H1 (F) was measured every 4 h in plants grown under standard conditions (LDs with 20/18 °C day/night temperatures). Grey-shaded areas indicate darkness. Expression levels were normalized to HvGAPDH and PTF. Error bars indicate the SE of the mean (n=3) of three biological replicates. Asterisks above lines indicate significant differences (two-sided Student’s t-test, *P<0.05, **P<0.01, and ***P<0.001).