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. 2023 Jun 27;12:e84822. doi: 10.7554/eLife.84822

Figure 4. Pupil-BOLD correlations based on systematic adjustment of the TTP of the HRF.

Graphs show the extracted t-statistics as a function of the systematically adjusted TTP for each participant in the subcortical ROIs (A) and the validation and control ROIs (D). p-Values refer to one-sample t-tests (difference from zero; FDR-corrected). Black dots indicate the mean. (B) Statistical maps showing pupil-BOLD correlations in the subcortex for the 1 s TTP. (c) Statistical maps showing unsmoothed pupil-BOLD correlations across the cortex for each TTP (1–6 s). All statistical maps were thresholded at p<0.005 (uncorrected) for visualization purposes only (n = 70). LC, locus coeruleus; VTA, ventral tegmental area; SN, substantia nigra; DR, dorsal raphe; MR, median raphe; BF, basal forebrain; ACC, anterior cingulate cortex; OCC, calcarine sulcus; BOLD, blood oxygen level-dependent; FDR, false discovery rate; TTP, time-to-peak; HRF, hemodynamic response function; ROI, region of interest. Source data used to generate this figure is available in Figure 4—source data 1.

Figure 4—source data 1. This folder contains the csv files and statistical maps used to make Figure 4.
Figure 4—source data 2. This contains the csv tables used in Figure 4—figure supplement 1.
Figure 4—source data 3. This folder contains the csv tables and statistical maps used in Figure 4—figure supplement 2.

Figure 4.

Figure 4—figure supplement 1. Pupil size-BOLD correlations based on systematic adjustment of the TTP of the HRF for both sessions separately.

Figure 4—figure supplement 1.

Graphs show the individual t-statistics (black point indicates the mean) of the pupil size-BOLD signal correlation as a function of the systematically adjusted TTP for all ROIs, p-Values refer to one-sample t-tests (difference from zero; FDR-corrected; n = 70). These results show that smaller ROIs, in particular the LC, DR, and MR, are more prone to a drop in statistical power when the data is halved. LC, locus coeruleus; VTA, ventral tegmental area; SN, substantia nigra; DR, dorsal raphe; MR, medial raphe; ACC, anterior cingulate cortex; OCC, calcarine sulcus; BOLD, blood oxygen level-dependent; FDR, false discovery rate; TTP, time-to-peak; HRF, hemodynamic response function; ROI, region of interest. Source data used to generate this figure is available in Figure 4—source data 2.
Figure 4—figure supplement 2. Pupil derivative-BOLD correlations based on systematic adjustment of the TTP of the HRF.

Figure 4—figure supplement 2.

(A) Graphs show the individual t-statistics (black point indicates the mean) of the pupil derivative-BOLD signal correlation as a function of the systematically adjusted TTP for all ROIs, p-Values refer to one-sample t-tests (difference from zero; FDR-corrected). (B) The corresponding whole-brain associations between BOLD signal and the pupil derivative as a function of the TTP of the HRF (1 s TTP: top panel to 6 s TTP: bottom panel). The warm colors reflect areas in which BOLD activity is positively correlated with pupil derivative. The cold colors reflect areas where BOLD activity is negatively correlated with the pupil derivative. Both positive and negative contrast maps were sampled at p<0.005 (uncorrected) for visualization only (n = 70). LC, locus coeruleus; VTA, ventral tegmental area; SN, substantia nigra; DR, dorsal raphe; MR, medial raphe; ACC, anterior cingulate cortex; OCC, calcarine sulcus; BOLD, blood oxygen level-dependent; FDR, false discovery rate; TTP, time-to-peak; HRF, hemodynamic response function; ROI, region of interest. Source data used to generate this figure is available in Figure 4—source data 3.