Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 May 25;19(7):887–899. doi: 10.1038/s41589-023-01344-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 5 — (a) Relative invasiveness of low-noise mNF-BACH1 (BL) and high-noise mNF-BACH1 (BH) clones at each Dox concentration with respect to the uninduced controls. Two-tailed t-test between uninduced control and every other dose, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001. (b) Relative invasiveness of low-noise mNF-GFP (GL) and high-noise mNF-GFP (GH) clones at each Dox concentration with respect to the uninduced controls. n = 3, one-way ANOVA, P > 0.05. (c) Synthetic mammalian Positive-Feedback (mPF) gene circuit for Dox-induced co-expression of reverse tetracycline-controlled transactivator (rtTA) and GFP::BACH1 fusion protein (mPF-BACH1). TetO: Tetracycline Operator; VP16: Virus Protein 16 transcription activator. (d) Left panel: Representative dose-responses of fluorescence intensity histograms from mPF-BACH1 integrated MB231 clone measured at 0, 50, 100, 150, 200, 250, 300, 400, 500, 800, 1000 and 2000 ng ml⁻¹ Dox levels, respectively. Right panel: Dose-responses of mean fluorescence intensity (MFI) for MB231 mPF-BACH1 clone (n = 3, Dox=10 corresponds to Dox=0). (e) Plotting the noise (CV) as a function of normalized mean gene expression for both low-noise mNF-BACH1 and mPF-BACH1 clones of MB231. Total four mean-noise decoupling pairs were selected based on the minimum mean differences between mNF and mPF populations. (f) Representative histograms of selected mean-noise decoupling pairs in (e). (g) Deconstruction of BACH1 expression distribution among mPF-BACH1 population in the context of invasion landscape. Invasiveness predictions for each dose were based on each subpopulation’s mean invasiveness in the context of invasion landscape in Fig. 3e. Mean Peak Ratio was averaged over 3 independent replicates. (h) Comparison of invasiveness for mPF versus mNF cells at four selected decoupled noise points along with mPF cell invasion predicted computationally. Two-tailed t-test for each experimental pair, n = 3, *P < 0.05, **P < 0.01.

Source data