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. 2023 Jun 7;27(4):525–535. doi: 10.1007/s40291-023-00656-z
Long-read DNA sequencing with an ONT nanopore sequencer successfully reads across the frequently mutated and notoriously hard to sequence ORF15 region of the RPGR gene, but with low yield.
Yield was increased using a flow cell wash kit.
The method described allows simultaneous sequencing of up to 24 samples in a single experiment, providing a rapid cost-effective protocol.