Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 28;14(6):380. doi: 10.1038/s41419-023-05897-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — A The schematic of the OIR model in mice. Exposure to hyperoxic (75% O₂) in mice from P7 to P12 induced the dropout of retinal vascularization. Ischemia insult triggered pathological neovascularization (NV) and peaked on P17. The NV regressed from P17 to P25. B Retina samples were collected from the normal and OIR groups of CX3CR1-GFP mice on P17. The expression of CX3CR1 (Green, GFP) on microglia was analyzed by IF staining. Scale bar, 100 µm (n = 4 per group). C The total retinal cells of the control and OIR group were analyzed by single-cell RNA-seq. D The top 20 genes upregulated and top ten genes downregulated in microglia of the OIR group were analyzed by R. Lgals3 was the most significant gene upgraded then. The trajectory analysis in the pseudotiome of microglia were presented in (E), showing the differentiation process by gradient colors and states in differentiation by different colors. F Ridge plot showed the activation and inflammation level of each state in trajectory analysis according to a set of genes. The expression of lgals3 in all the seven states of microglia was shown in (G). H, I IF staining of retinal vessels (CD31, red), microglia (CX3CR1, GFP), and Gal3 (blue) on P17 in the flat-mounted cross-section from normal and OIR groups. Scale bar, 100 µm (n = 3 per group). Bars = means ± SD; **P < 0.01; ***P < 0.001.