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. 2023 Jun 28;14(6):380. doi: 10.1038/s41419-023-05897-8

Fig. 2. Removal of microglia and conditional knockout of Gal3 in microglia alleviate retinal angiogenesis.

Fig. 2

A The schematic of PLX5622 administration in the OIR mice by gavage from P12 since removed to normoxic condition. B The number of CX3CR1+ microglia were evaluated by FACS and quantified in the normal, OIR, and PLX group (n = 3 per group). C IF staining of microglia (CX3CR1, GFP) and Gal3 (blue) in the flat-mounted retinal tissue from the Ctr OIR group and Plx OIR group showed the removal of microglia in the retina by the administration of Plx5622. (n = 3 per group) Scale bar, 100 µm. D IF staining was used to evaluate vessel clusters (CD31, red) and avascular areas. (n = 3 per group). Scale bar, 500 µm. E The whole retinas of CX3CR1-tdT-Lgals3(f/f, f/−. −/−) mice were collected and the vessels were stained with CD31 (red). (n = 3 per group). Scale bar, 500 µm. The specific morphology of blood vessels was captured in (F). (n = 3 per group). Scale bar, 100 µm. Bars = means ± SD; *P < 0.05; **P < 0.01; ***P < 0.001, NS no significance.