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. 2023 Jun 28;14(6):380. doi: 10.1038/s41419-023-05897-8

Fig. 3. Gal3 regulated mainly on ECs rather than on microglia.

Fig. 3

BV2 microglia were cultured in hypoxia for 12 h, followed by immunostaining for IBA1(A). Scale bar, 100 µm. B, C mRNA expression of M1-related genes (iNOS, CD86, TNF-α, and IL-6) and M2-related genes (Arg1, CD206, IL-4, and IL-10) were determined by qRT-PCR (n = 3 per group). D HUVECs cultured by conditioned media collected from BV2 microglia in normoxia and hypoxia for 12 h, followed by immunostaining for Ki67 (red). (n = 7 per group). Scale bar, 100 µm. E HUVECs were wounded with a p20 pipette tip and then cultured by conditioned media from BV2 microglia in normoxia and hypoxia for 12 h. Photographs were taken immediately and after 8 h (n = 7 per group for wound assay and two fields were calculated per sample). Scale bar, 100 µm.Bars = means ± SD; *P < 0.05; **P < 0.01; ***P < 0.001, NS no significance.