Fig. 6.

Synthetic GCT over-represented eccMIRs benefited cell growth and facilitated aggressive features of host cells. A Cell viability assay of MGC803 cells after sole eccMIR or eccMIR-mix transfection. B–D Flow cytometry-based apoptosis detection of MGC803 cells after sole eccMIR or eccMIR-mix transfection. B Dot plots of eccMIRs transfection groups. C Cells ratio of early-phase-apoptosis in each group. D Cells ratio of late-phase-apoptosis in each group. E–H Transwell migration and matrigel invasion assays of MGC803 cells after eccMIRs transfection. E Images represent the cells that invaded into the membrane in each group. F Average number of invaded cells in each group. G Average number of migrated cells in each group. H Images displayed the migrated cells in either synthetic eccRandom or eccMIR548w transfected group. I Relative ratio of EdU positive cells in each group after synthetic eccMIR transfection. J Images presented the clonogenic abilities of MGC803 cells 7 days after synthetic eccRandom, eccMIR-mix and eccMIR4725 transfection. K Colonies formation number in each group after synthetic eccMIRs transfection. Mean and S.E. of three or four experiments. ns indicated no significant differences; *P < 0.05, **P < 0.01 and ***P < 0.001 in a Student’s t test