Fig. 3.

COX/NOX activity, via ROS production, is involved in NFkB-dependent VCAM1 and ICAM1 expression increase in extracellular histone-treated HUVEC. A Protein extracts (20 µg protein) from HUVEC exposed to different concentrations of histones for 4 h (n = 4) were loaded on SDS-PAGE gels and analyzed by Western blotting using anti-p-p65, anti-p65, and anti-NLRP3. β-actin was used as loading control. One representative experiment of three performed is shown. Relative levels assessed by densitometry are presented. B HUVECs were incubated with Bay11-7082 (Bay) and exposed to 50 µg/mL of histones for 4 h (n = 4). Relative VCAM1 and ICAM1 expression were determined by qRT-PCR. C HUVECs were incubated with tempol, apocynin, and celecoxib, and exposed to 50 µg/mL of histones for 4 h (n = 3). Relative VCAM1 and ICAM1 expression were determined by qRT-PCR. Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001 versus 0 µg/mL of histones. ^#P < 0.05, ^##P < 0.01 versus 50 µg/mL of histones