REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Anti-NeuN | Millipore | MAB377X; RRID:AB_2149209 |
Anti-RBPMS | Novus Biological | NBP2-20112 |
Biological samples | ||
Frozen adult post-mortem human retinas | UTAH LIONS Eye Bank | N/A |
Chemicals, peptides, and recombinant proteins | ||
Rnase inhibitor | Takara Bio | 2323B |
MACS BSA Stock Solution | Miltenyi Biotec | 130-091-376 |
Protease inhibitor | Roche | 11697498001 |
Tween 20 | Bio-Rad | 1662404 |
Trizma Hydrochloride Solution, pH 7.4 | Sigma-Aldrich | T2194 |
Sodium Chloride Solution, 5M | Sigma-Aldrich | 59222C |
Magnesium Chloride Solution, 1M | Sigma-Aldrich | M1028 |
Nonidet P40 Substitute IGEPAL CA-630 | Sigma-Aldrich | I8896 |
Critical commercial assays | ||
Chromium Next GEM Single Cell 3′ Kit v3.1 | 10x Genomics | |
Chromium Next GEM Single Cell ATAC Library & Gel Bead Kit v1.1 | 10x Genomics | |
Deposited data | ||
snRNA-seq and snATAC-seq data | This paper |
https://data.humancellatlas.org/explore/projects/9c20a245-f2c0-43ae-82c9-2232ec6b594f https://cellxgene.cziscience.com/collections/af893e86-8e9f-41f1-a474-ef05359b1fb7 |
Software and algorithms | ||
Cell Ranger | 10x Genomics | 3.0.2 |
Cell Ranger ATAC | 10x Genomics | 1.2.0 |
SoupX | Young and Behjati.40 | https://github.com/constantAmateur/SoupX |
Seurat | Hao et al.41 | https://satijalab.org/seurat/ |
DoubletFinder | McGinnis et al.42 | https://github.com/chris-mcginnis-ucsf/DoubletFinder |
scVI | Lopez et al.43 | https://scvi-tools.org/ |
scPred | Alquicira-Hernandez et al.44 | https://github.com/powellgenomicslab/scPred |
LIGER | Welch et al.36 | https://github.com/welch-lab/liger |
Pysam | https://pysam.readthedocs.io/en/latest/api.html | |
Bedtools | https://bedtools.readthedocs.io/en/latest/ | |
macs2 | Zhang et al.45 | https://pypi.org/project/MACS2/ |
Deeptools | Ramírez et al.46 | https://deeptools.readthedocs.io/en/develop/ |
SCENIC | Aibar et al.35 | https://github.com/aertslab/SCENIC |