Skip to main content
. 2023 Apr 11;3(6):100298. doi: 10.1016/j.xgen.2023.100298
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Anti-NeuN Millipore MAB377X; RRID:AB_2149209
Anti-RBPMS Novus Biological NBP2-20112

Biological samples

Frozen adult post-mortem human retinas UTAH LIONS Eye Bank N/A

Chemicals, peptides, and recombinant proteins

Rnase inhibitor Takara Bio 2323B
MACS BSA Stock Solution Miltenyi Biotec 130-091-376
Protease inhibitor Roche 11697498001
Tween 20 Bio-Rad 1662404
Trizma Hydrochloride Solution, pH 7.4 Sigma-Aldrich T2194
Sodium Chloride Solution, 5M Sigma-Aldrich 59222C
Magnesium Chloride Solution, 1M Sigma-Aldrich M1028
Nonidet P40 Substitute IGEPAL CA-630 Sigma-Aldrich I8896

Critical commercial assays

Chromium Next GEM Single Cell 3′ Kit v3.1 10x Genomics
Chromium Next GEM Single Cell ATAC Library & Gel Bead Kit v1.1 10x Genomics

Deposited data

snRNA-seq and snATAC-seq data This paper https://data.humancellatlas.org/explore/projects/9c20a245-f2c0-43ae-82c9-2232ec6b594f
https://cellxgene.cziscience.com/collections/af893e86-8e9f-41f1-a474-ef05359b1fb7

Software and algorithms

Cell Ranger 10x Genomics 3.0.2
Cell Ranger ATAC 10x Genomics 1.2.0
SoupX Young and Behjati.40 https://github.com/constantAmateur/SoupX
Seurat Hao et al.41 https://satijalab.org/seurat/
DoubletFinder McGinnis et al.42 https://github.com/chris-mcginnis-ucsf/DoubletFinder
scVI Lopez et al.43 https://scvi-tools.org/
scPred Alquicira-Hernandez et al.44 https://github.com/powellgenomicslab/scPred
LIGER Welch et al.36 https://github.com/welch-lab/liger
Pysam https://pysam.readthedocs.io/en/latest/api.html
Bedtools https://bedtools.readthedocs.io/en/latest/
macs2 Zhang et al.45 https://pypi.org/project/MACS2/
Deeptools Ramírez et al.46 https://deeptools.readthedocs.io/en/develop/
SCENIC Aibar et al.35 https://github.com/aertslab/SCENIC