Figure 2.

Characteristics of HCC and immune infiltrates

(A) Volcano plot from differential abundance analysis (two-tailed Welch’s t test) between proteomes of non-tumorous (n = 33) and tumorous liver tissue (n = 32). Each dot represents a protein. Signature proteins of HCC tumors identified in a previous proteomics study²² are indicated in dark blue. See also Figure S2A and Table S2.

(B) Heatmap showing the abundance (Z score) of the five most strongly up- and downregulated proteins in HCC tissue in individual patients.

(C) Differential analysis of the arginine and proline metabolism between non-tumorous and tumorous tissue. Enzymes are color-coded according to the fold change as determined by the differential abundance analysis in (A).

(D) FACS-purified CD4⁺ and CD8⁺ T cells, CD14⁺ monocytes/macrophages, as well as NK cells from blood, liver, and tumor tissue were analyzed by LC-MS. The plots show the number of identified proteins in each sample.

(E) Boxplots showing the abundance of CD4, CD8, CD56, and MRC1 protein in different cell types isolated from blood, liver, and tumor tissue. Each dot represents a sample from a different patient. See also Figure S2C.