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. Author manuscript; available in PMC: 2023 Oct 20.
Published in final edited form as: Mol Cell. 2022 Oct 10;82(20):3856–3871.e6. doi: 10.1016/j.molcel.2022.09.019

Figure 4. Depletion of mRNP maturation factors affecting basket assembly and Mlp1 localization.

Figure 4.

A. Depletion screen for changes in Mlp1 localization (120 min post-auxin); changes indicated by arrows. B. Distribution of Mlp1-GFP in Rna14AID-HA and Rna15AID-HA cells. C. Mlp1-GFP distribution with respect to nucleolus in strains with altered perinuclear Mlp1-GFP signal. Line scan intensity plot show comparative Mlp1-GFP and Gar1-tdTomato distribution; arrows indicate Mlp1 signal at nucleolar periphery. Scale bar =2μm.