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. Author manuscript; available in PMC: 2023 Oct 20.
Published in final edited form as: Mol Cell. 2022 Oct 10;82(20):3856–3871.e6. doi: 10.1016/j.molcel.2022.09.019

Figure 5. Baskets assemble on a subset of NPCs in the nucleoplasm and co-localize with select proteins at the nuclear periphery.

Figure 5.

A. SIM of Nup188-GFP or Mlp1-GFP and Gar1-tdTomato; signal intensity distribution shown in line plots and circular diagrams where red dashes delimit nucleolar region; average background signal shown as grey circles and GFP distribution in green. B. Signal distribution analysis of selected GFP-tagged Nups or Mlp1-GFP compared to Nup188-tdTomato along the nucleoplasmic periphery; each dot represents the occupied proportion/cell (in %; n=100). C. SIM co-localization analysis of Nop1-CFP, Nup188-tdTomato, and Nup42-, Nup1-, Nup133- or Mlp1-GFP. D. SIM co-localization analysis of Nup188-tdTomato and Sac3-, Ulp1-, Pml39- and Mex67-GFP. E. SIM co-localization analysis of Mlp1-Halo and Sac3-, Ulp1-, Pml39- and Mlp2-GFP. F. SIM co-localization analysis of Mlp1-GFP and Sac3-, Ulp1-, and Pml39-Halo at 30°C or 42°C. G. SIM co-localization analysis of Mlp1-GFP and Sac3-, Ulp1-, Pml39-, and Nab2-Halo in Rbp2AID-HA cells pre- and 120 min post-auxin. Scale bar =2μm.