Figure 1.

BAI3-deletion mice were generated using CRISPR/Cas9. (A) Coding exons for the full-length and seven predicted BAI3 transcripts. (B) CRISPR/Cas9 targeted BAI3^+/+ exon 2 and exon 18 for generating BAI3^−/− mice. Genotyping by PCR to show mutations in exon 2 and exon 18 of BAI3. (C) Western blots show protein expression in the whole brain and muscle lysates isolated from BAI3^+/+ and BAI3^−/− mice (n = 4). Actin was used as a loading control. (D) The tissue expression profile of BAI3 was determined by PCR using primers designed to differentiate between the cDNA obtained from BAI3^+/+ and BAI3^−/− mice. Actin was used as a loading control to show the presence of DNA (n = 3).