Table 4.
Detection of LSD DNA by real-time PCR in blood, incubation period, days of viremia, IPMA, ELISA and clinical diagnosis of the acceptor animals.
| ID Acceptor Animal | Fed on Donor Animal | First Day Flies on (dpi) | Incubation Period: Min-Max (Days) * | Peak Ct in Blood (dpf) | Viraemic Period (dpf) |
Nodules Present | IPMA | ELISA | Clinical Diagnosis |
|---|---|---|---|---|---|---|---|---|---|
| A1 | preclinical D10 | 10 dpi | / | / | / | no | neg | neg | non-infected |
| A2 | preclinical D7 | 7 dpi | / | / | / | no | neg | neg | non-infected |
| A3 | preclinical D7 | 7 dpi | / | / | / | no | neg | neg | non-infected |
| A4 | preclinical D7 | 7 dpi | / | / | / | no | neg | neg | non-infected |
| A5 | preclinical D10 | 7 dpi | / | / | / | no | neg | neg | non-infected |
| A6 | preclinical D10 | 7 dpi | / | / | / | no | neg | neg | non-infected |
| A7 | preclinical D10 | 10 dpi | / | / | / | no | neg | neg | non-infected |
| A8 | preclinical D10 | 7 dpi | / | / | / | no | neg | neg | non-infected |
| A9 | subclinical D2 | 15 dpi | / | / | / | no | pos | neg | undetermined |
| A10 | subclinical D2 | 15 dpi | / | / | / | no | neg | neg | non-infected |
| A11 | subclinical D2–D4 | 15 dpi | / | / | / | no | neg | neg | non-infected |
| A12 | subclinical D4 | 15 dpi | 13–27 | 34.44 (50) | 27, 43 until 57 | no | pos | pos | subclinical |
| A13 | subclinical D4 | 15 dpi | 6–20 | 31.59 (22) | 20 until 36 | yes | pos | pos | clinical |
* Since flies were fed several times on donors and acceptors and the exact occasion at which transmission occurred remains unknown, the incubation period in the acceptor animals is indicated by minimum or maximum number of days.