Figure 1.

Schematic representation of the procedure in the ECT+GET treatment group. The tumor is represented with a red circle. The first step is to inject on the periphery of the tumor 200 μg of plasmid encoding canine IL-2, represented with a yellow circle, and then deliver the GET pulses. The second step is to inject 200 μg of plasmid encoding canine IL-12 in the quadriceps muscle (yellow circle) and then deliver the GET pulses. The third step is to treat with ECT using intravenous BLM, the tumor, and the safety margins (green area). Twenty-eight days later, steps 1 and 2 are repeated.