Figure 13.

The phylogram based on a Maximum Likelihood analysis of combined LSU, SSU, ITS and tef1-α sequence datasets. Related sequences were taken from Mortimer et al. [71]. The analyzed gene contains 23 fungal strains and 3280 total characters including gaps (LSU: 1–860 bp, SSU: 861–1903 bp, ITS: 1904–2388 bp, tef1-α: 2389–3280 bp). The tree topology of the ML resembles BI. The matrix had distinct alignment patterns, with the final ML optimization likelihood value of −11,066.133147 (ln). All free model parameters were estimated by the RAxML model, with 822 distinct alignment patterns and 43.17% of undetermined characters or gaps. Estimated base frequencies were as follows: A = 0.234980, C = 0.257617, G = 0.290520 and T = 0.216883, with substitution rates AC = 1.135176, AG = 1.801869, AT = 1.203774, CG = 1.318707, CT = 7.467159 and GT = 1.000000. The gamma distribution shape parameter alpha = 0.211437, and the Tree-Length = 1.143995. The final average standard deviation of split frequencies at the end of total MCMC generations was calculated as 0.009737 in BI analysis. The type strains are denoted in bold with the symbol “✱” at the ends, and newly introduced species in this study are denoted in red. The nodes provide bootstrap values of at least 60% (ML, left) and Bayesian posterior probabilities of at least 0.95 (BI, right); hyphens (-) signify values that are less than 60% in ML and less than 0.95 in BI. The greenish and pale brown backgrounds were used to distinguish different genera groups, while the yellow background indicates the genus Heleiosa.