. 2023 Jun 16;9(6):680. doi: 10.3390/jof9060680

Table 1.

The PCR conditions and primers used in this study.

Locus	Definition	Primer	Annealing (PCR)	Reference
ITS	Internal transcribed spacer	ITS4	^a 55 °C, 50 s ^b	[20]
ITS	Internal transcribed spacer	ITS5	^a 55 °C, 50 s ^b	[20]
LSU	Large subunit 28S	LR0R	^a 55 °C, 50 s ^b	[21]
LSU	Large subunit 28S	LR5	^a 55 °C, 50 s ^b	[21]
rpb2	DNA-dependent RNA polymerase II largest subunit	5F	^a 57 °C, 55 s ^b	[22]
rpb2	DNA-dependent RNA polymerase II largest subunit	7cR	^a 57 °C, 55 s ^b	[22]
SSU	The partial18S small subunit	NS1	^a 55 °C, 50 s ^b	[20]
SSU	The partial18S small subunit	NS4	^a 55 °C, 50 s ^b	[20]
tef1-α	Translation elongation factor 1 alpha	983F	^a 56 °C, 50 s ^b	[23]
tef1-α	Translation elongation factor 1 alpha	2218R	^a 56 °C, 50 s ^b	[23]
tub2	Beta-tubulin 2	Bt2a	^a 52 °C, 50 s ^b	[24]
tub2	Beta-tubulin 2	Bt2b	^a 52 °C, 50 s ^b	[24]

^a Initial denaturation of 2 min at 94 °C, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing temperature and time for different genes are mentioned in Table 1. ^b 90 s of extension at 72 °C and a final extension of 10 min at 72 °C.