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. 2023 Jun 14;11(6):1573. doi: 10.3390/microorganisms11061573

Figure 1.

Figure 1

Growth analysis of MSMEG_4272 knock-down strain. (a) MSMEG_4272-ID fusion protein is stable in the absence of HIV-2 protease. In the presence of HIV-2 protease, the protecting peptide is removed and protein degraded through proteolysis. The MSMEG_4272 protein levels were analysed by western blotting using (b) anti-FLAG to evaluate ssrA-tag exposure and (c) anti-myc antibodies to determine protein degradation. Aliquots from (1) 4272ID and (2) 4272ID::HIV2Pr, with and without Atc, were taken after 16 h, 25 h, and 40 h of growth (dot-dash lines). Protein quantity for western blotting analysis was standardized by protein concentration using the Bradford assay. (d) Growth monitored over 43 h for the M. smegmatis strains: 4272ID and 4272ID::HIV2Pr with (open square and circle) and without (solid square and circle) the addition of Atc, respectively. Values represent the mean, and error bars represent standard deviation for three independent experiments.