Figure 2.

Growth of M. smegmatis strain 4272ID::sgRNA. (a) Culturing of the 4272ID::sgRNA strain in the presence and absence of Atc (solid circle and open circle, respectively). Data represent the mean of three biological replicates, and error bars indicate standard deviation. (b) Western blotting with anti-FLAG antibody was used to determine MSMEG_4272 protein levels for 4272ID::sgRNA after 45 h. Bradford assays were used to standardise the total protein loaded for western blotting. Higher molecular weight bands represent multimers of the protein due to incomplete reduction.