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. 2023 Jun 14;11(6):1573. doi: 10.3390/microorganisms11061573

Figure 5.

Figure 5

Growth for CRISPRi mediated MSMEG_4272 gene silencing in M. smegmatis strains under iron-limiting culture conditions. (a) Growth of WT::sgRNA, in MM media without iron (dashed line) or with 2 µM iron (solid line), in the presence (solid triangle) or absence (open triangle) of Atc. (b) Growth of 4272ID::empty in MM media without iron (dashed line) or with 2 µM iron (solid line), in the presence (solid square) or absence (open square) of Atc. (c) Growth of 4272ID::sgRNA in MM media without iron (dashed line) or with 2 µM iron (solid line), in the presence (solid circle) or absence (open circle) of Atc. The dot dash line indicates the time point of Atc addition to appropriate cultures. (d) Comparison of 4272ID::sgRNA in MM media with 2 µM iron (solid line, as previously represented in Figure 5c) and 7H9 (dashed line, as previously represented in Figure 3b), in the presence (open circle) or absence (solid circle) of Atc. Data represent the mean of three biological replicates, and error bars indicate standard deviation.