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. 2023 May 12;3(6):1623–1633. doi: 10.1021/jacsau.3c00084

Figure 2.

Figure 2

(a) Traces comparing the binding to ApoE R2 of the two different protein orientations grafted on the NPs (NP-CT and NP-NT with about 400 proteins/NPs). Constructs were injected at 20 and 200 μg/mL (injection happening between the green and red arrows) without regeneration of the surface between the 2 injections. The results are shown in duplicate. (b) Investigation of specificity of NP-ApoE construct interactions with ApoER2 surfaces. Constructs at a concentration of 20 μg/mL were pre-mixed with free ApoE R2 at increasing concentrations and injected onto an immobilized ApoER2. Total suppression of interaction was achieved with 9 μg/mL of free receptor. (c–e) Binding profiles obtained for NPs with grafted (NP-CT) and adsorbed ApoE (NP-corona) on immobilized ApoE R2 (∼20 Hz) (c), LDLR (∼60 Hz) (d), or MARCO (∼40 Hz) (e). Constructs were injected at 20 and 200 μg/mL (injection happening between the green and red arrow) without regeneration of the surface between the 2 injections.