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. 2023 Jun 18;11(6):1111. doi: 10.3390/vaccines11061111

Figure 3.

Figure 3

Cytopathic effect (CPE) and RT-PCR products of the rescued and parental FMDVs at 24 h post-infection in BHK-21 cells. (A) The rescued FMDVs of both types A (NP05) and O (O198) produced CPE similarly to the corresponding parental viruses while mock-infected cells (control) did not develop CPE. (B) Electrophoretic photographs demonstrated the RT-PCR products amplified with common FMDV (FMDV 2B) and type-specific (VP1) primers. The FMDV 2B primers produced an approximately 420 bp DNA fragment, while the FMDV type-specific primers amplified the VP1 gene region and generated 630 and 714 bp DNA fragments for rNP05 and rO189, respectively. Lanes 1–4 are RT-PCR products amplified with FMDV 2B primers; control [1], rescued O189 [2], rescued NP05 [3], and wild-type FMDV [4]. Lanes 5 and 6 are PCR products amplified using cDNA from the rescued and parental O189 viruses, respectively, with OVP1 primers. Lanes 7 and 8 are PCR products amplified with AVP1 primers using cDNA from the rescued and parental NP05 viruses, respectively.