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. 2023 Jun 18;11(6):1111. doi: 10.3390/vaccines11061111

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunoperoxidase monolayer assay (IPMA) demonstrates specific reactivity between wild-type (WT) or rescued FMDVs and a monoclonal antibody (mAb) raised against FMDV (3E11). FMDV types O (O189) and A (NP05), and rescued virus types O (rO198) and A (rNP05) infected BHK-21 cells reacted strongly with the mAb 3E11 as shown by the brown color. mAb 3E11 did not bind to non-infected BHK-21 cells and porcine circovirus type 2 (PCV2) infected PK-15 cells, which served as 3E11 background and isotype controls, respectively. Both WT and rescued FMDVs did not react to mAb 12G3, a mAb specific to PCV2 [26]. PCV2-infected PK-15 cells show a positive signal with mAb 12G3, while non-infected PK-15 cells remained negative. (Magnification of 100×).