Table 1.
Strains used in the study.
| Strains | Mutation Description | Source |
|---|---|---|
| Bacillus subtilis WT 168 | Natural isolate (wild-type) | Proffessor J. Stuelke, University of Göttingen, Germany |
| Protease-deficient strains | ||
| Bacillus subtilis BG20-36 | ΔnprE–522; Δapr–684 | Eugenio Ferrarri, Genencor Int. Inc., Rochester, NY, USA |
| Bacillus subtilis BRB08 | ΔtrpC2, ΔnprB, ΔaprE, Δepr, Δbpr, ΔnprE, Δmpr, Δvpr, ΔwprA | Cobra Biologics, Keele, UK |
| Bacillus subtilis BRB14 | ΔtrpC2, ΔnprB, ΔaprE, Δepr, Δbpr, ΔnprE, Δmpr, Δvpr, ΔwprA, ΔhtrA, ΔhtrB | Cobra Biologics, Keele, UK |
| Strains with regulatory mutations | ||
| Bacillus subtilis 168 abrB::kan (amyE::pAT606) | ΔabrB (Kan) | Dr. Prof. T. Masher Ludwig-Maximillian’s University, Munich, Germany |
| Bacillus subtilis 168 degU::kan (amyE::pAT606) | ΔdegU (Kan) | Dr. Prof. T. Masher Ludwig-Maximillian’s University, Munich, Germany |
| Bacillus subtilis 168 spo0A::tet (amyE::pAT612) | Δspo0A (Tet) | Dr. Prof. T. Masher Ludwig-Maximillian’s University, Munich, Germany |