(A) the cell count in HGF as it was mentioned cells was examined by flow cytometry. (B) Shows the MTT assay with different concentrations of probiotic bacteria after 12 and 24 h. As depicted in the chart, our probiotic supernatant did not affect the HGF-1 cells significantly in comparison to the control group. All the values are expressed as means ± SD (n = 3) and ns = not significant, compared with control group. Figures C show the cell count in HGF by QH, QFSC, QSSC and QFSC/SSC. (QH = quadrant Health population, QFSC=quadrant Forward side scatter population (shown cell had change shape), QSSC = quadrant Side scatter population (shown cell had change granulate)).