Table 1.
shows the cells percentage population after 6, 24 and 48 h for exposure to different concentrations of probiotic supernatant (I, II, III are 3.62, 7.8 and 10.68 mg/mL, respectively). As you can see MCF-7 cells stay alive after exposure to probiotic supernatant for all concentrations after 6 and 24 h, whereas the viability of MCF-7 cells after 48 h decreased and showed dose concentration and time dependency compared with control group at each time of concentration. All the values are expressed as percentage. ns = not significant, *P < 0.05,**P < 0.01, ***P < 0.001and ****P < 0.0001 compared with control group.
| Apoptosis/Necrosis assay% | |||||
|---|---|---|---|---|---|
| Cell-Time/ (mg/ml) | Concentration | Control | I | II | III |
| Live cell | 6h | 99.72 | 97.45 ns | 99.53ns | 97.77** |
| 24h | 98.35 | 98.89 ns | 98.95ns | 99.07** | |
| 48h | 99.51 | 11.93*** | 28** | 4.14**** | |
| Necrotic cell | 6h | 0.02 | 2.47* | 21**** | 1.07* |
| 24h | 1.25 | 0.93* | 0.04**** | 0.45* | |
| 48h | 0.1 | 0.00* | 16.25**** | 40.25**** | |
| Apoptotic cell | 6h | 0.16 | 0.00* | 0.12* | 0.43*** |
| 24h | 0.26 | 0.06*** | 0.97** | 0.27** | |
| 48h | 0.19 | 42.16**** | 6.42** | 0.01* | |