Fig. 3.

Patient-specific iPSCs take the lead for precision medicine in LQTS. Blood samples are collected for generation of patient-specific iPSCs. Pathogenic genetic variants are identified through whole-genome sequencing. CRISPR/Cas9 mediated genome editing can either introduce or correct a mutation in patient-specific iPSCs to study genotype-phenotype interactions. Deep phenotyping of LQTS iPSC-derived cardiomyocytes include multielectrode arrays, pharmacological profiling, electrophysiological characterisation and immunocytochemistry. Preclinical testing in LQTS patient-specific cardiomyocytes will guide clinicians for precise stratification and treatment of LQTS.