Table 1.
Chemical characterization of peptide conjugates.
| Sequence | Rt | Mcalc | Mmeas |
|---|---|---|---|
| Cf-Arg8 | 11.6 | 1624.14 | 1623.86 |
| Acyl-Met-(Arg)6-Met-(Gly)4-Lys(Cf) | 12.8 | 1971.95 | 1971.97 |
| Dabcyl-Met-(Arg)6-Met-(Gly)4-Lys(Cf) | 15.0 | 2181.05 | 2181.10 |
| Acyl-Cys((Arg)6-Cys)-(Gly)4-Lys(Cf) | 11.9 | 1913.88 | 1913.77 |
| Dabcyl-Cys((Arg)6-Cys)-(Gly)4-Lys(Cf) | 13.8 | 2122.97 | 2123.01 |
| Dabcyl-Cys-(Arg)2-Cys-(Arg)2-Cys-Lys(Cf) TBMB | 15.9 | 1801.75 | 1801.70 |
| Dabcyl-Cys-(Arg)3-Cys-(Arg)3-Cys-Lys(Cf) TBMB | 14.4 | 2113.95 | 2113.85 |
| Dabcyl-Cys-(Arg)4-Cys-(Arg)4-Cys-Lys(Cf) TBMB | 13.9 | 2426.16 | 2425.55 |
| Acyl-Cys-(Arg)2-Cys-(Arg)2-Cys-Lys(Cf) TBMB | 12.5 | 1592.66 | 1592.28 |
| Acyl-Cys-(Arg)3-Cys-(Arg)3-Cys-Lys(Cf) TBMB | 12.0 | 1904.86 | 1904.44 |
| Acyl-Cys-(Arg)4-Cys-(Arg)4-Cys-Lys(Cf) TBMB | 11.4 | 2217.06 | 2217.07 |
| Dabcyl-Cys-(Gly)4-Cys-(Arg)6-Lys(Cf) para BBMB | 14.7 | 2227.03 | 2227.10 |
| Dabcyl-Cys-(Gly)4-Cys-(Arg)6-Lys(Cf) orto BBMB | 14.8 | 2227.03 | 2227.07 |
| Dabcyl-Cys-(Arg)6-Cys-(Gly)4-Lys(Cf) para BBMB | 14.2 | 2227.03 | 2227.01 |
| Dabcyl-Cys-(Arg)6-Cys-(Gly)4-Lys(Cf) orto BBMB | 14.1 | 2227.03 | 2226.99 |
| Dabcyl-(Arg)6-Cys-(Gly)4-Cys-Lys(Cf) para BBMB | 13.6 | 2227.03 | 2226.94 |
| Dabcyl-(Arg)6-Cys-(Gly)4-Cys-Lys(Cf) orto BBMB | 13.5 | 2227.03 | 2227.05 |
| Acyl-Cys-(Gly)4-Cys-(Arg)6-Lys(Cf) para BBMB | 12.2 | 2017.94 | 2017.94 |
| Acyl-Cys-(Gly)4-Cys-(Arg)6-Lys(Cf) orto BBMB | 12.4 | 2017.94 | 2017.90 |
| Acyl-Cys-(Arg)6-Cys-(Gly)4-Lys(Cf) para BBMB | 12.4 | 2017.94 | 2017.88 |
| Acyl-Cys-(Arg)6-Cys-(Gly)4-Lys(Cf) orto BBMB | 12.2 | 2017.94 | 2017.94 |
| Acyl-(Arg)6-Cys-(Gly)4-Cys-Lys(Cf) para BBMB | 12.4 | 2017.94 | 2017.86 |
| Acyl-(Arg)6-Cys-(Gly)4-Cys-Lys(Cf) orto BBMB | 12.6 | 2017.94 | 2017.95 |
| Dabcyl-Cys-(Arg)6-Cys-(Gly)4-Lys(DauSuc) para BBMB | 16.4 | 2477.97 | 2478.08 |
| Dabcyl-Cys-(Arg)6-Cys-(Gly)4-Lys(DauSuc) orto BBMB | 16.5 | 2477.97 | 2478.13 |
The analytical chromatogram was recorded using Hypersil Hypurity C18 column (4.6 mm × 150 mm, 5 μm, 190 Å) with linear gradient elution (0 min 0% B, 2 min 0% B, 22 min 90% B) at 1 mL/min flow rate. The absorbance was measured at λ = 220 nm. The Bruker Amazon SL (Germany) mass spectrometer was used to perform the mass spectrometric analysis. The samples were dissolved in acetonitrile–water (50:50, v/v) containing 0.1% formic acid.