Figure 1.

MTS cell viability assay. (A) Percent proliferation of the MBA-MB-231 cells after 24 and 48 h of treatment with different concentrations of UD (0–5% v/v). Results are compared to those of the untreated cells. Significant growth inhibition was observed on treatment with UD (p < 0.001). The IC₅₀ was found to be 6.459% v/v at 24 h and 4.161% v/v at 48 h. (B) Percent proliferation of cells after 24 and 48 h of treatment with different concentrations of cisplatin (5–25 μM). A decrease in the proliferation of MDA-MB-231 cells was observed with increasing cisplatin concentrations. The IC₅₀ was found to be 32.21 μM at 24 h and 12.76 μM at 48 h (p < 0.001). (C) Percent proliferation of cells upon treatment with increasing cisplatin concentrations in combination with 4% v/v UD after 24 h, (D) and 48 h. Results are compared to those of the cells treated with cisplatin alone. Each value represents the mean ± SD from three separate experiments; statistical analysis was determined by using Student’s t-test with * p < 0.5, ** p < 0.05, *** p < 0.001.