Table 1.
Experimental plan for obtaining immobilized α-amylase *.
| Sample Code * | α-Amylase Concentration, mg/mL | Magnesium Acetate Concentration, % | Immobilization Efficiency, Ef% | Enzymatic Activity/g of Particles·mL |
|---|---|---|---|---|
| A1 | 1 | 2 | 66.7 ± 4.8 | 3.97 ± 0.3 |
| A2 | 2 | 2 | 68.45 ± 6.6 | 4.36 ± 0.05 |
| A3 | 3 | 1 | 63.49 ± 1.6 | 4.71 ± 0.05 |
| A4 | 3 | 2 | 75.56 ± 5.1 | 4.28 ± 0.15 |
| A5 | 3 | 3 | 89.68 ± 3.1 | 4.15 ± 0.05 |
| A6 | 3.5 | 2 | 66.89 ± 0.6 | 3.83 ± 0.1 |
| A7 | 4 | 2 | 66.07 ± 0.6 | 4.63 ± 0.05 |
| A8 | 5 | 2 | 56.9 ± 1.7 | 3.66 ± 0.05 |
* Concentration of the gellan solution: 1%; pH of the acetate buffer solution used: 5.6; immobilization temperature: 60 °C.