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. Author manuscript; available in PMC: 2024 Apr 28.
Published in final edited form as: Science. 2023 Apr 27;380(6643):382–387. doi: 10.1126/science.add7328

Fig. 2. RAD51 recombinase activity promotes fork reversal.

Fig. 2.

(A-C) Fork protection assays were completed in U2OS cells expressing near endogenous levels of the indicated RAD51 proteins after transfection with the indicated siRNAs. P values were calculated using a Kruskal-Wallis test. (EV, empty vector) (D-E) Replication elongation rate in the presence of 50nM camptothecin (CPT) in D) or 150mM cisplatin in E). P values were calculated using a Kruskal-Wallis test. (F) Percentage of reversed replication forks in U2OS cells expressing endogenous levels of the indicated RAD51 proteins. Cells were transfected with the indicated siRNA and treated 72 hours later with 4mM HU, 20μM MRE11 inhibitor (Mirin) and 25μM DNA2 inhibitor (C5) for 5 hours. The number of replication intermediates analyzed for each condition is indicated in parentheses and a representative image is shown (right panel), P: parental strand, D: daughter strand and R: reversed arm.