Schematic representation of the genomic organization and features of ANV-5/IM162/19 strain (upper panel) compared to other ANVs (tabulated in the lower panel). The coordinates and sizes (nt) of ORF1a, ORF1b and ORF2 (encoding nsP, RdRp and CP, respectively) are indicated. Other features shown include trypsin-like serine peptidase (TLSP), nuclear localization signal (NLS), ribosomal shift sequence (RFS), stem-loop II-like motif (s2m), 5′-/3′-unstranalated regions (UTR), and nine of the variable regions (A–I) that have been reported in ORF2 of ANVs and other AstVs [5,13,17,31,35,50]. Sequence names include GenBank accession numbers, abbreviated host avian species, and country/strain/strain/year of isolation.