Tissue distribution of 12 ILs in healthy T. obscurus. The transcripts of ToIL-1β, ToIL-1, ToIL-6, ToIL-10, ToIL-11, ToIL-12, ToIL-17, ToIL-18, ToIL-20, ToIL-24, ToIL-27, and ToIL-34 were measured by RT-qPCR. β-actin was used as an internal control. The tissues, including heart, liver, kidney, spleen, gills, and intestine were collected from three individuals. Three biological repeats were set in each group. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)