Fig. 5.

Relative expression levels of 12 ILs in the spleen of T. obscurus at different time points (0, 12, 24, 36, 48, and 72 h) as measured with RT-qPCR after S. aureus and V. harveyi infection. β-actin was employed as an internal control. Each experiment was performed in triplicate. Data are shown as mean ± S.D. Asterisks indicate significant differences (*P < 0.05, **P < 0.01, ***P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)