Table 1.
Sequences of the primers used in the study.
| Primers name | Sequences (5′−3′) | Tm ( °C) |
|---|---|---|
| ToIL-1β-F | ATGGAATCTCAGATGAAATCCAACG | 63.6 |
| ToIL-1β-R | TTACATCTCTCCCTCACACGTGGGCG | 72.1 |
| ToIL-1-F | ATGGACCTGAAGGAATCAGAGGTA | 61.1 |
| ToIL-1-R | TCATTTCTTAATGACAAAGAGGAAGG | 60.8 |
| ToIL-6-F | ATGGCTTCCATCAGTTACCTGCTCGC | 70.7 |
| ToIL-6-R | TCACGATCTCTTCTTTGGGTGGTAG | 63.8 |
| ToIL-10-F | ATGACTCCCGGCTCTCTGCTCTCCG | 72.8 |
| ToIL-10-R | TCAGGCCACGTTGCGGCGCCTCTTT | 79.3 |
| ToIL-11-F | ATGAAATTGCTCCTCGACTCCTCCTC | 67.5 |
| ToIL-11-R | TCATTGCACCGCGGCATTGACCTTGG | 78.5 |
| ToIL-12-F | ATGGCCTCGCTCCACCTCTGTGAGTA | 71.2 |
| ToIL-12-R | TCAGCTGCTGGGCTGAGCATCGGCAC | 78.6 |
| ToIL-17-F | ATGCAGGTGGTGTCGGGAACGCTGCT | 77.0 |
| ToIL-17-R | GGCCCACTGTTATACCCCAACAGTGA | 69.2 |
| ToIL-18-F | ATGGCAGCTAACAATGGCAATTTTG | 66.6 |
| ToIL-18-R | CTAAGACATGGTGACGTAGCAGGTC | 62.2 |
| ToIL-20-F | ATGAAGACTCTGTCATCCCTCTGCCT | 65.9 |
| ToIL-20-R | TCAGTTCGGACTTTTCTGCTCGGAG | 68.1 |
| ToIL-24-F | ATGCTCGCCTCCACACTCCGCTGCTC | 77.0 |
| ToIL-24-R | TCAGGCCACGGTAACGTTGGTCTGG | 72.8 |
| ToIL-27-F | ATGATTGGGTGGACCACTCTGGCTCT | 70.1 |
| ToIL-27-R | CTACAGATTGCTTTTGGGTAAAGTGAT | 62.1 |
| ToIL-34-F | ATGTGCACGCCCCTGAAGACCATCAA | 74.1 |
| ToIL-34-R | TCACACACGTAAGGTGGGCGCCGCGT | 79.8 |
| ToIL-1β-qF | ATGAGCATGCAGGAGTACAG | 53.1 |
| ToIL-1β-qR | GGTTCACTTTGCGACATTCC | 57.8 |
| ToIL-1-qF | AACACGTGGCCTCTCATTAC | 54.9 |
| ToIL-1-qR | CTCCTCCTCATTTGCCTTGTAG | 58.5 |
| ToIL-6-qF | CGACTTTCTGGACCGTTACAA | 57.9 |
| ToIL-6-qR | CCGCTTGAGAAGAACTGAGTAG | 56.5 |
| ToIL-10-qF | TTCTACCTGGACTCGGTTCT | 53.7 |
| ToIL-10-qR | TGGTCGAAGATCTGCTGAATG | 58.5 |
| ToIL-11-qF | GTCGGCCCATAGTCTCAATAATC | 59.8 |
| ToIL-11-qR | CCTGTTCAGCCACTCAAAGT | 54.9 |
| ToIL-12-qF | GAGGTGGAAGAAGAGAAGGTTG | 57.4 |
| ToIL-12-qR | ACAGCTCTGTTGATGGTGATG | 55.4 |
| ToIL-17-qF | TACTATCCTACGCTGGTCCTC | 54.0 |
| ToIL-17-qR | GTCCTCAGCTGGAACTCATATT | 55.4 |
| ToIL-18-qF | GAGAATCACAGAAGGGTGCTATC | 57.5 |
| ToIL-18-qR | GGATGACTTTGTTCAGCGTTTG | 59.9 |
| ToIL-20-qF | ACGTAGAGAGAGTGTTCAGGAG | 52.9 |
| ToIL-20-qR | CGCAGAGGCAGTGACATTTA | 57.1 |
| ToIL-24-qF | GCCCAACATCAAGCCAAATC | 60.2 |
| ToIL-24-qR | CAGCACGTTGTTCAGGTAGA | 54.2 |
| ToIL-27-qF | TGCTGGTGCGTCAGTTATC | 54.7 |
| ToIL-27-qR | CAGACTCTGTGTGTCTCTCTCT | 50.2 |
| ToIL-34-qF | TATCCAGGAGACTTGGGATCA | 56.8 |
| ToIL-34-qR | ATGGTGTAGCACAGGTTGTC | 52.6 |
| β-actin-qF | GCATTGTCACCAACTGGGATG | 60.8 |
| β-actin-qR | GCAGGACTGGATGCTCCTCT | 58.9 |