Fig. 2.

ALKBH5 attenuated proliferation, migration, and invasion of CRC cells. a-b qRT-PCR and western blot analysis of ALKBH5 mRNA and protein levels after overexpression ALKBH5 in HCT116 and SW620. c-d CCK8 was performed to detect the proliferation of CRC cells transfected with ALKBH5 plasmid compared with vector. e-f The colony formation assay provides representative images and quantification of ALKBH5 or vector-transfected HCT116 and SW620 cells. g-h The migratory and invasive capacities of ALKBH5 overexpressed HCT116 and SW620 cells were assessed using transwell migration and invasion assays. All data were indicative of three separate studies and shown as mean ± SD. Student's t-test. The Student's t-test was used to determine P values.