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Translation of viral proteins and viral RNA replication in HAstV-1 UTR deletion mutants. (a) BSR-T7 cells transfected with wt HAstV-1 and deletion mutants. The HAstV capsid protein is labeled in red and cellular nuclei in blue. Scale bar = 20 µm. (b) Images from the in situ hybridization for antigenomic HAstV-1 RNA of BSR-T7 cells transfected with wt HAstV-1 and deletion mutants. Red dots and arrows indicate RNA probe hybridization. Scale bar = 50 µm, 40× magnification.
